Difference between revisions of "TopHat 2016"

From HPC users
Jump to navigationJump to search
(Created page with "== Introduction == TopHat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes, and then analyzes the mapping results to iden...")
 
 
(2 intermediate revisions by the same user not shown)
Line 4: Line 4:
== Installed version ==
== Installed version ==


The currently installed version is  
The currently installed version is available on both environments ''hpc-env/6.4'' and ''hpc-uniol-env'':
  TopHat/'''2.1.1-intel-2018a'''


== Using TopHat ==


== Using XXXXXXXXXX ==
If you want to find out more about TopHat on the HPC Cluster, you can use the command


If you want to find out more about XXXXXXXXXXX on the HPC Cluster, you can use the command
  module spider TopHat
 
  module spider XXXXXXXXXXXXX


This will show you basic informations e.g. a short description and the currently installed version.
This will show you basic informations e.g. a short description and the currently installed version.
Line 17: Line 17:
To load the desired version of the module, use the command, e.g.
To load the desired version of the module, use the command, e.g.


  module load  XXXXXXXXXXXXXXXXXx
  module load hpc-env/6.4
  module load TopHat


Always remember: this command is case sensitive!
Always remember: this command is case sensitive!


''You might consider using [[HISAT2 2016 | HISAT2]] instead of TopHat, because HISAT2 provides the same core functionalities as TopHat, but it is considered more effective and accurate.''


== Documentation ==
== Documentation ==


The full documentation can be found [ here].
The full documentation can be found [https://ccb.jhu.edu/software/tophat/manual.shtml here].

Latest revision as of 14:31, 28 June 2019

Introduction

TopHat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes, and then analyzes the mapping results to identify splice junctions between exons.¹

Installed version

The currently installed version is available on both environments hpc-env/6.4 and hpc-uniol-env:

 TopHat/2.1.1-intel-2018a

Using TopHat

If you want to find out more about TopHat on the HPC Cluster, you can use the command

module spider TopHat

This will show you basic informations e.g. a short description and the currently installed version.

To load the desired version of the module, use the command, e.g.

module load hpc-env/6.4
module load TopHat

Always remember: this command is case sensitive!

You might consider using HISAT2 instead of TopHat, because HISAT2 provides the same core functionalities as TopHat, but it is considered more effective and accurate.

Documentation

The full documentation can be found here.