IsoSeq3 2016

Introduction

IsoSeq v3 contains the newest tools to identify transcripts in PacBio single-molecule sequencing data. Starting in SMRT Link v6.0.0, those tools power the IsoSeq GUI-based analysis application. A composable workflow of existing tools and algorithms, combined with a new clustering technique, allows to process the ever-increasing yield of PacBio machines with similar performance to IsoSeq versions 1 and 2. Starting with version 3.4, support for UMI and cell barcode based deduplication has been added. ¹

Installed version(s)

The following versions are installed and currently available...

... on environment hpc-env/8.3:

• name/version-GCC-8.3.0

Loading / Using IsoSeq3

To load the desired version of the module, use the module load command, e.g.

module load hpc-env/8.3
module load IsoSeq3 

Always remember: this command is case sensitive!

If you want to find out more about IsoSeq3 on the HPC cluster, you can use the command

module spider IsoSeq3 

This will show you basic information e.g. a short description and the currently installed version.

Documentation

The documentation can be found here. Information about clustering can be found here And information about deduplication can be found here