Bcl2fastq2

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Introduction

The Illumina sequencing instruments generate per-cycle base call (BCL) files at the end ofthe sequencingrun. A majority of analysis applications use per-read FASTQ files as input for analysis. bcl2fastq can be used to convert base call (BCL) files from a sequencingrun into FASTQ files.

bcl2fastq supports the following instruments:

  • MiniSeq
  • MiSeq
  • NextSeq 500, 550
  • HiSeq X
  • HiSeq 2000, 2500, 3000, 4000
  • NovaSeq 5000, 6000

Installed version

The currently installed version of bcl2fastq is 2.18.0.12.

Documentation

The full documentation for bcl2fasatq can be found here.