Difference between revisions of "GeneMark-EX 2016"

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== Installed version ==
== Installed version ==
The GeneMark bundle is installed on the environment ''hpc-env/6.4:
The GeneMark bundle is installed on the environment ''hpc-env/6.4:
'''Genemark-EX/4.33-intel-2018a-Perl-5.26.0'''
'''Genemark-EX/4.33-intel-2018a-Perl-5.26.0'''



Revision as of 10:30, 1 October 2018

Introduction

GeneMark-EX is a bundled installation of GeneMark-ES and GeneMark-ET.


Installed version

The GeneMark bundle is installed on the environment hpc-env/6.4:

Genemark-EX/4.33-intel-2018a-Perl-5.26.0

Using GeneMark-EX on the HPC cluster

To load GeneMark-EX type in:

module load hpc-env/6.4
module load Genemark-EX


To run GeneMark-ES:

gmes_petap.pl --ES --sequence seq.fna 
gmes_petap.pl --ES  --fungi --sequence seq.fna    

To run GeneMark-ET, provide i/genomic sequence and ii/ RNA-Seq read mapped intron coordinates in GFF format:

gmes_petap.pl --sequence seq.fna --ET introns.gff  --et_score 4

You should adjust command line parameter "--et_score", depending on the type of RNA-Seq read alignment tool used.

Documentation

If you need more information about GeneMark, you can visit their website.