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In bioinformatics, BLAST (Basic Local Alignment Search Tool) is an algorithm for comparing primary biological sequence information, such as the amino-acid sequences of proteins or the nucleotides of DNA sequences. A BLAST search enables a researcher to compare a query sequence with a library or database of sequences, and identify library sequences that resemble the query sequence above a certain threshold.

Installed version

These versions are installed and and currently available...

... on envirnoment hpc-uniol-env:

  • BLAST/2.2.26
  • BLAST/2.6.0
  • BLAST+/2.7.1-intel-2016b-Python-2.7.12

... on environment hpc-env/6.4:

  • BLAST+/2.7.1-intel-2018a
  • BLAST+/2.7.1-foss-2017b-Python-2.7.14

... on environment hpc-env/8.3:

  • BLAST/2.11.0-Linux_x86_64
  • BLAST+/2.11.0-gompi-2019b
  • BLAST+/2.13.0-gompi-2019b

Available Commands

BLAST offers three categories of applications:

  • search tools: blastn, blastp, blastx, tblastx, tblastn, psiblast, rpsblast, rpstblastn
  • BLAST database tools: makeblastdb, blastdb_aliastool, makeprofiledb, blastdbcmd
  • sequence filtering tools: segmasker, dustmasker, windowmasker


If you want to find out more about BLAST on the HPC Cluster, you can use the command

module spider blast

This will show you basic informations e.g. a short description and the currently installed version.

To load the desired version of the module, use the command, e.g.

module load BLAST/2.6.0-Linux_x86_64 

Always remember: this command is case sensitive!


The full documentation can be found here.

Direct link to the command line manual (PDF viewer required): BLAST® Command Line Applications User Manual